Coding

Part:BBa_K1027002:Design

Designed by: Robert Harrison, Lorna Hepworth, Vun Hiang Tan   Group: iGEM13_Manchester   (2013-09-19)


Delta 12 Desaturase, optimised for expression in Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
    Illegal BamHI site found at 426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 247
    Illegal AgeI site found at 511
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for expression in E coli


Source

Produced synthetically based on protein sequence from Synechocystis sp.

References

Li MC, Li H, Wei DS, Xing LJ 2006. Cloning and molecular characterization of Δ12-fatty acid desaturase gene from Mortierella isabellina. World J Gastroenterol 12(21): 3373-3379

Sakamoto, T.; Bryant, D.A. 2002. Synergistic effect of high-light and low temperature on cell growth of the Δ12 fatty acid desaturase mutant in Synechococcus sp. PCC 7002. Photosynth. Res.,72, 231-242

Yadav, Narendra S., Quinn Qun Zhu, and Hongxiang Zhang 2009 .Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast. U.S. Patent No. 7,504,259.